SECOND-GENERATION MULTIPLEX IMMUNOASSAYS xMAP Technology and Magnetic Appeal Put Multiplexing in the Fast Lane
ثبت نشده
چکیده
منابع مشابه
Development and validation of a multiplex add-on assay for sepsis biomarkers using xMAP technology.
BACKGROUND Sepsis is a common and often fatal disease. Because sepsis can be caused by many different organisms, biomarkers that can aid in diagnosing sepsis and monitoring treatment efficacy are highly warranted. New sepsis markers may provide additional information to complement the currently used markers. METHODS We used a combination of in-house and commercially available multiplex immuno...
متن کاملBio-Rad’s Bio-Plex® suspension array system, xMAP technology overview
The Bio-Plex(®) system utilizes xMAP technology to permit the multiplexing of up to 100 different analytes. Multiplex analysis gives researchers the ability to look at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods. Similar to ELISA, xMAP utilizes an antibody sandwich for detection but differs from ELISA in capture su...
متن کاملxMAP Technology: Applications in Detection of Pathogens
xMAP technology is applicable for high-throughput, multiplex and simultaneous detection of different analytes within a single complex sample. xMAP multiplex assays are currently available in various nucleic acid and immunoassay formats, enabling simultaneous detection and typing of pathogenic viruses, bacteria, parasites and fungi and also antigen or antibody interception. As an open architectu...
متن کاملCombined serology and antibiotic residue detection in a Luminex assay
Serum of slaughter pigs is being used for routine testing of antibodies against pathogens and can also be used for detection of antibiotic residues. Since the assay formats differ (direct detection of antibodies vs. inhibition assays for residues), these tests are performed in parallel, not in a multiplex set-up. For this study, a protocol for testing both antibodies and residues in one sample ...
متن کاملAutomated Workflows for Luminex xMAP Assays
Bead-based, multiplexing assays such as those provided by the Luminex xMAP technology, have proven to be highly useful for biomarker identification and quantification from cells, tissues and body fluids. Applications include biological research, drug discovery and clinical diagnostics. The original technology based on the use of polystyrene microspheres tends to have arduous, manual work flows ...
متن کامل